Purification and characterization of extracellular exo-chitosanase from soil fungus, Penicillium sp. IB-37-2

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Doklady Bashkirskogo Universiteta. 2017. Volume 2. No. 3. pp. 380-385.

Authors

Bogdanova Yu. G.
Bashkir State University
32 Zaki Validi Street, 450074 Ufa, Republic of Bashkortostan, Russia
Yangildina Yu. Ya.
Ufa State Petroleum Technological University
1 Kosmonavtov Street, 450062 Ufa, Republic of Bashkortostan, Russia
Teregulova G. A.
Ufa Institute of Biologys, Russian Academy of Sciences
69 Prospekt Oktyabrya, 450054 Ufa, Republic of Bashkortostan, Russia
Aktuganov G. E.*
Ufa Institute of Biologys, Russian Academy of Sciences
69 Prospekt Oktyabrya, 450054 Ufa, Republic of Bashkortostan, Russia

Abstract

The novel exo-chitosanase with Mw 41 kDa was isolated from culture supernatant of chitosan-degrading fungus, Penicillium sp. IB-37-2 and characterized. Using the procedures of ultrafiltration, affinity adsorption and hydrophobic chromatography 43-fold purification of the enzyme was reached. The main physic-chemical and catalytic properties of the chitosanase were studied.

Keywords

  • chitosanase
  • exo-β-D-glucosaminidase
  • Penicillium
  • purification
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