Creation of microbial-plant communities for phytoremediation

Doklady Bashkirskogo Universiteta. 2018. Volume 3. No. 4. pp. 473-476.

Authors


Khakimova L. R.*
Institute of Biochemistry and Genetics UFRC RAS
71 Oktyabrya Avenue, 450054 Ufa, Republic of Bashkortostan, Russia
Blagova D. K.
Institute of Biochemistry and Genetics UFRC RAS
71 Oktyabrya Avenue, 450054 Ufa, Republic of Bashkortostan, Russia
Lavina A. M.
Institute of Biochemistry and Genetics UFRC RAS
71 Oktyabrya Avenue, 450054 Ufa, Republic of Bashkortostan, Russia
Serbaeva E. R.
Bashkir State University
32 Zaki Validi Street, 450074 Ufa, Republic of Bashkortostan, Russia
Sadykova L. R.
Institute of Biochemistry and Genetics UFRC RAS
71 Oktyabrya Avenue, 450054 Ufa, Republic of Bashkortostan, Russia
Vershinina Z. R.
Institute of Biochemistry and Genetics UFRC RAS
71 Oktyabrya Avenue, 450054 Ufa, Republic of Bashkortostan, Russia
Baimiev Al. Kh.
Institute of Biochemistry and Genetics UFRC RAS
71 Oktyabrya Avenue, 450054 Ufa, Republic of Bashkortostan, Russia

Abstract


Phytoremediation is an environmentally friendly and inexpensive method for cleaning soils contaminated by heavy metals (HM). For phytoremediation using superaccumulater plants capable of accumulating HM is promising. The using of synthetic pseudophytochelatine genes ( pph ) can potentially increase the availability of HM for plants. The article describes the creation of the vector pCambia1305.1- pph6 for obtaining of transgenic model tobacco plants. As a result, at 100 μM concentration of cadmium in the soil, transformed plants expressing the pph6 gene accumulated cadmium 35% more than the control plants.

Keywords


  • phytoremediation
  • heavy metals
  • cadmium
  • pph6 gene