Construction of plasmid for expression of horseradish peroxidase gene isoenzyme C2 in yeast cells
Doklady Bashkirskogo Universiteta. 2018. Volume 3. No. 5. pp. 509-513.
Authors
Martynava E. A.
Institute of Microbiology of the National Academy of Sciences of Belarus
2 Kuprevicha Street, 220141 Minsk, Belarus
Semashko T. V.*
Institute of Microbiology of the National Academy of Sciences of Belarus
2 Kuprevicha Street, 220141 Minsk, Belarus
*E-mail: tsemashko@mbio.bas-net.by
Valentovich L. N.
Institute of Microbiology of the National Academy of Sciences of Belarus
2 Kuprevicha Street, 220141 Minsk, Belarus
Zhukovskaya L. A.
Institute of Microbiology of the National Academy of Sciences of Belarus
2 Kuprevicha Street, 220141 Minsk, Belarus
Abstract
Codon optimization of horseradish peroxidase Armoracia rusticana gene isoform C2 (hrpC2) for expression in yeast cells was carried out. On the basis of plasmid pPICZαA vector containing synthesized hrpC2 was constructed and transformants of Pichia pastoris were obtained.
Keywords
- horseradish peroxidase gene isoform C2
- vector for gene expression
- transformation
- Escherichia coli
- Pichia pastoris